1. Sheldon Cooper, Ph.D - The Big Bang Theory
2. Veronica Mars - Veronica Mars
3. Jaye Tyler - Wonderfalls
4. Landry Clarke - Friday Night lights
5. Coach Eric Taylor - Friday Night Lights
6. Barney Stinson - How I Met Your Mother
7. Georgia "George" Lass - Dead Like Me
8. Emerson Cod - Pushing Daisies
9. Tammy Taylor - Friday Night Lights
10. Benjamin "Ben" Linus aka Henry Gale - Lost
11. Colonel John Casey - Chuck
12. Michael Bluth - Arrested Development
13. Edward "Ed" Stevens - Ed
14. Albert "Al" Swearengen - Deadwood
15. Richard "Dick" Casablancas - Veronica Mars
16. Thomas Michael "Tommy" Gavin - Rescue Me
17. Dr. Harold Abbott Jr. - Everwood
18. Jayne Cobb - Firefly
19. Earl Jehosophat Hickey - My Name Is Earl
20. Hugo "Hurley" Reyes - Lost
21. Rockefeller Butts - The Knights of Prosperity
22. Lucius Vorenus - Rome
23. Penny - The Big Bang Theory
24. Olive Snook - Pushing Daisies
25. Sean Leslie Garrity - Rescue Me
26. Julius - Everybody Hates Chris
27. Eugene Gurkin - The Knights of Prosperity
28. Dexter Morgan - Dexter
29. Captain Malcolm "Mal" Reynolds - Firefly
30. Buddy Garrity - Friday Night Lights
31. Franco Rivera - Rescue Me
32. Bill Haverchuck - Freaks and Geeks
33. Charles "Chuck" Bartowski - Chuck
34. Special Agent Sarah Walker - Chuck
35. Lieutenant Kenneth "Lou" Shea - Rescue Me
36. Keith Mars - Veronica Mars
37. Joy Farrah (Hickey) Turner (née Darville) - My Name Is Earl
38. Andrew Wells - Buffy the Vampire Slayer
39. Chloe Sullivan - Smallville
40. Sheriff Don Lamb - Veronica Mars
41. Krevlornswath "Lorne" of the Deathwok Clan - Angel
42. Kaywinnit Lee "Kaylee" Frye - Firefly
43. Sherriff Seth Bullock - Deadwood
44. George Bluth Sr. - Arrested Development
45. Winifred "Fred" Burkle - Angel
46. Daisy Adair - Dead Like Me
47. Mike Silletti - Rescue Me
48. Chris - Everybody Hates Chris
49. Carol Vessey - Ed
50. Brian "Smash" Williams - Friday Night Lights
51. Frannie Morgan - Greek
52. Lester Patel - Chuck
53. Dr. Daniel Faraday - Lost
54. Lucille Bluth - Arrested Development
55. Darnell "Crabman" Turner aka Harry Monroe - My Name Is Earl
56. Joan Girardi - Joan of Arcadia
57. King Silas Benjamin - Kings
58. Tyra Collette - Friday Night Lights
59. Dwight Kurt Shrute III - The Office
60. Jonathan "Jack" Donahue Bristow - Alias
61. Eli Cartwright Goggins - Ed
62. Sayid Jarrah - Lost
63. Warren P. Cheswick - Ed
64. Richard "Rick" Castle - Castle
65. Tobias Fünke - Arrested Development
66. Bart "Black Shawn" Johnston - Rescue Me
67. Louise Brooks - Gary Unmarried
68. Detective Katherine "Kate" Beckett - Castle
69. Gourishankar "Gary" Subramaniam - The Knights of Prosperity
70. Titus Pullo - Rome
71. Dale Kettlewell - Greek
72. Margaret "Meg" Pryor - American Dreams
73. Maggie Dekker - Eli Stone
74. Jeffery "Jeff" Barnes - Chuck
75. Ned "The Piemaker" - Pushing Daisies
76. Devin "Captain Awesome" Woodcomb, M.D. - Chuck
77. Lyla Garrity - Friday Night Lights
78. James "Sawyer" Ford - Lost
79. Rebecca Logan - Greek
80. Johnathan "John" Locke aka Jeremy Bentham - Lost
81. George Oscar "Gob" Bluth Jr. - Arrested Development
82. Matt Saresen - Friday Night Lights
83. Miles Straume - Lost
84. Eli "Weevil" Narvarro - Veronica Mars
85. Alec Hardison - Leverage
86. Wendy Watson - The Middleman
87. Casey Cartwright - Greek
88. Charles Gunn - Angel
89. Hannah Rogers - Everwood
90. Francis "Squatch" Squacieri - The Knights of Prosperity
91. "Big" Mike Tucker - Chuck
92. Shirley Pifko - Ed
93. Cindy "Mac" McKenzie - Veronica Mars
94. Maeby Fünke - Arrested Development
95. Rochelle - Everybody Hates Chris
96. Harvey Lipschultz - Boston Public
97. Lana Lang - Smallville
98. Detective Eddie Arlette - Keen Eddie
99. Leonard Leakey Hofstadter, Ph.D - The Big Bang Theory
100. Harold Brighton "Bright" Abbott - Everwood
(Scooter’s Note: Only characters that were introduced and featured for at least one full season between September 1999 and August 2009 were eligible for this list. For a complete breakdown on the list, check out Breaking Down the 100 Greatest Television Characters of the 00's.)